The objective of present study was to develop Stevia rebaudiana as a sweetener in pharmaceutical formulation. The major constituents are stevioside and rebaudioside A. The leaves of stevia are the source of diterpene glycosides, viz. stevioside and rebaudioside. Stevioside is regenerated as a valuable natural sweetening agent because of its good taste and chemical stability. It has both economical and medicinal importance. Sugar is the most popular sweetener in the world. However due to adverse health effects of sucrose and known artificial sweeteners, interest increased in search for no calorie natural sweeteners has been intensified in recent years. Isolation of stevioside in purest form dried stevia leaves powder by different extraction method. Isolated stevioside was analysed and characterized by various spectral, chromatographic and physicochemical method. It was also compared with standard stevioside with all analytical methods. Stevioside also showed synergistic antidiabetic activity in type 2 diabetes with antidiabetic drug gliclazide. Its suspension was prepared by employing complex processing steps like wetting, suspending, thickening, flocculating agent and preservative where stevioside was used as sweetener for masking metallic taste of gliclazide. Optimized formulation batch of Suspension was evaluated with evolutionary parameters such as sedimentation rate, sedimentation volume, particle size, viscosity, zeta potential and also evaluated for in-vitro drug release study. The optimized batch showed drug release up to 98% in 1 hour. The formulation was stable during thermodynamic study. The formulation showed good stability. Confirmed by in-vitro drug release and viscosity determination. Stevioside showed good compatibility with all excipient of suspension.
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